免疫细胞培养专用细胞因子

巨噬细胞(Macrophage)通常被认为是由骨髓中的造血干细胞到髓系祖细胞到单核细胞发育而来,迁移到组织后分化为组织特异性巨噬细胞,它存在两个不同的亚群:M1型(经典激活)和M2型(替代激活)。M1型巨噬细胞具有促炎作用,能被脂多糖(LPS)单独或与Th1细胞因子(如IFN-γ,GM-CSF)联合极化,并产生促炎细胞因子,如IL-1β、IL-6、IL-12、IL-23和TNF-α;M2型巨噬细胞具有抗炎和免疫调节作用,能被Th2细胞因子IL-4和IL-13极化,产生IL-10和TGF-β等抗炎细胞因子。M1/M2巨噬细胞的平衡极化对于维系机体健康至关重要。

巨噬细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human CSF-1/M-CSF ProteinHuman1435C-HisHEK293 cellsRP01221
Recombinant Mouse CSF-1/M-CSF ProteinMouse12977No-TagHEK293 cellsRP01216S
Recombinant Rat CSF-1/M-CSF ProteinRat78965C-HisHEK293 cellsRP01847
Recombinant Human CSF-2/GM-CSF ProteinHuman1437C-HisHEK293 cellsRP00094
Recombinant Mouse CSF-2/GM-CSF ProteinMouse12981N-HisHEK293 cellsRP01206
Recombinant Rat CSF-2/GM-CSF ProteinRat116630N-HisHEK293 cellsRP01207
Recombinant Human IFN-gamma ProteinHuman3458No tagE. coliRP01038
Recombinant Mouse IFN-gamma ProteinMouse15978C-hFc&HisHEK293 cellsRP01070
Recombinant Rat IFN-gamma ProteinRat25712C-hFc&HisHEK293 cellsRP01075
Recombinant Human IL-4 ProteinHuman3565C-HisHEK293 cellsRP00995
Recombinant Mouse IL-4 ProteinMouse16189C-HisHEK293 cellsRP01161
Recombinant Rat IL-4 ProteinRat287287C-HisHEK293 cellsRP01874
Recombinant Human IL-13 ProteinHuman3596C-HisHEK293 cellsRP01320
Recombinant Mouse IL-13 ProteinMouse16163C-HisHEK293 cellsRP01596
Recombinant Human TNF-alpha ProteinHuman7124C-HisHEK293 cellsRP00993
Recombinant Mouse TNF-alpha ProteinMouse21926C-HisHEK293 cellsRP01071
Recombinant Rat TNF-alpha ProteinRat24835C-HisHEK293 cellsRP01322
Recombinant Human IL-10 ProteinHuman3586C-HisHEK293 cellsRP00093
Recombinant Mouse IL-10 ProteinMouse16153C-HisHEK293 cellsRP01465
Recombinant Rat IL-10 ProteinRat25325C-HisHEK293 cellsRP03252
LPS (Lipopolysaccharides)Escherichia coli 055:B5///RPM0001
PMA (佛波酯) (PKC激活剂)16561-29-8/固体C36H56O8RPM0015

巨噬细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Mouse CSF-1/M-CSF stimulates cell proliferation of the M-NFS-60 cells.
Recombinant Mouse IL-4 stimulates cell proliferation of the HT-2 cells
Recombinant Mouse IL-10 promote cell proliferation of MC/9 mouse mast cells
客户培养数据展示
浙江大学
Experiment Type
Cell-based assay
Sample
Mouse bone marrow cells
Product
Recombinant Mouse CSF-1/M-CSF Protein (RP01216)
Description
Mouse bone marrow cells were taken and cultured in a cell culturedish for 3 hours. Following this, the cells were transferred to a bacteria culture dish and cultured inmedium containing 20 ng/mL M-CSF, The medium was changed every 3 days, and after 7 days, the cells were matured, Flow cytometrywas employed to assess changes in macrophage markers, revealing a positive rate of macrophages as high as 98%. Additionally, results from phagocytosis experiments indicated that the induced macrophages exhibited strong phagocytic capability.
中国人民解放军陆军军医大学第一附属医院
Experiment Type
Cell-based assay
Sample
Mouse bone marrow cells
Product
Recombinant Mouse CSF-1/M-CSF Protein (RP01216); Recombinant Mouse IL-4 Protein (RP01161)
Description
1、Extract the bone marrow cells of a 8-week-old male C57 mouse, stimulate the cells with mouse CSF-1 (Cat: RP01216) at a final concentration of 20 ng/mL for one week (change the culture mediumevery day). Collect the cells, and detect the result of macrophage induction by flow cytometry.

2.Stimulate the M0 cells with mouse IL-4 (Cat. RP01161) at a final concentration of 40 ng/mL. Then collect the cells detect the result of M2 polarization by flow cytometry.

树突状细胞(Dendritic cell, DC)是一类来源于骨髓造血系统的异质性细胞,可由髓系祖细胞和淋巴祖细胞发育而来,是目前发现的功能最强的抗原呈递细胞(APC)。DC细胞能在天然免疫中识别病原体,在适应性免疫中通过MHC分子呈递抗原肽来诱导Naïve T细胞激活和分化,是先天免疫和适应性免疫之间的主要桥梁。在常规的DC诱导培养中,GM-CSF、IL-4和TNF-α被认为是最重要的三大细胞因子,其中GM-CSF促进DC生成;IL-4通过抑制DC前体细胞向巨噬细胞分化,而分化为未成熟的DC;加入TNF-α后,DC前体细胞即向成熟表型DC分化。

树突状细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human CSF-2/GM-CSF ProteinHuman1437C-HisHEK293 cellsRP00094
Recombinant Mouse CSF-2/GM-CSF ProteinMouse12981N-HisHEK293 cellsRP01206
Recombinant Rat CSF-2/GM-CSF ProteinRat116630N-HisHEK293 cellsRP01207
Recombinant Human Flt3 ligand/Flt3L ProteinHuman2323C-HisHEK293 cellsRP00175
Recombinant Mouse Flt3 ligand/Flt3L ProteinMouse14256C-hFc&HisHEK293 cellsRP01058
Recombinant Human IL-4 ProteinHuman3565C-HisHEK293 cellsRP00995
Recombinant Mouse IL-4 ProteinMouse16189C-HisHEK293 cellsRP01161
Recombinant Rat IL-4 ProteinRat287287C-HisHEK293 cellsRP01874
Recombinant Human IL-6 ProteinHuman3569C-HisHEK293 cellsRP00201
Recombinant Mouse IL-6 ProteinMouse16193C-HisHEK293 cellsRP01321
Recombinant Rat IL-6 ProteinRat24498C-HisHEK293 cellsRP01741
Recombinant Human IL-1 beta ProteinHuman3553C-HisE. coliRP00002
Recombinant Mouse IL-1 beta ProteinMouse16176C-HisE. coliRP01340
Recombinant Rat IL-1 beta ProteinRat24494C-HisHEK293 cellsRP01788
Recombinant Human TNF-alpha ProteinHuman7124C-HisHEK293 cellsRP00993
Recombinant Mouse TNF-alpha ProteinMouse21926C-HisHEK293 cellsRP01071
Recombinant Rat TNF-alpha ProteinRat24835C-HisHEK293 cellsRP01322
LPS (Lipopolysaccharides)Escherichia coli 055:B5///RPM0001

树突状细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Mouse CSF-2/GM-CSF stimulates cell proliferation of FDC-P1 cells
Recombinant Human CSF-2/GM-CSF stimulates cell proliferation of FDC-P1 cells
Recombinant Mouse IL-4 stimulates cell proliferation of the HT-2 cells
客户培养数据展示
厦门大学
Experiment Type
Cell-based assay
Sample
Mouse bone marrow cells
Product
Recombinant Mouse IL-4 Protein (RP01161)
Description
C57BL/6 mouse bone marrow cells were induced with IL-4 (10 ng/mL, Cat. RP01161) and GM-CSF (20 ng/mL) to differentiate into bone marrow derived dendritic cells (BMDC). At 5 days and 6 days after induction, the expression of CD11c and MHC Ⅱ were detected by flow cytometry to analyze the maturation of BMDC.
中山大学
Experiment Type
Cell-based assay
Sample
Mouse bone marrow cells
Product
Recombinant Mouse Flt3 ligand/Flt3L Protein (RP01058)
Description
Recombinant Mouse FLT3L(80 ng/mL) induce C57BL/6 mouse bone marrow cells to differentiate into BMDC. After 12 days, the maturity of dendritic cells was measured by flow cytometry.

自然杀伤细胞(Natural killer cell,NK细胞)来源于骨髓造血干细胞,属于先天免疫系统的核心细胞,由共同淋巴祖细胞发育而来,也是除T细胞和B细胞之外的第三大类淋巴细胞。NK细胞主要分布于外周血、肝脏和脾脏,通过激活和抑制信号的控制来识别和杀死靶细胞。由于具有无MHC限制的自然杀伤活性、通过分泌IFN-γ等细胞因子和趋化因子参与免疫调节、能介导抗体依赖细胞介导的细胞毒性作用(ADCC),NK细胞常被用于开发肿瘤免疫细胞疗法。在体外培养过程中常用IL-2、IL-12、IL-15、IL-21等细胞因子刺激NK细胞,提高其活性。

NK细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human IL-2 ProteinHuman3558C-HisHEK293 cellsRP01039
Recombinant Mouse IL-2 ProteinMouse16183C-HisHEK293 cellsRP01384
Recombinant Rat IL-2 ProteinRat116562C-HisHEK293 cellsRP01769
Recombinant Human IL-7 ProteinHuman3574C-HisHEK293 cellsRP01040
Recombinant Mouse IL-7 ProteinMouse16196C-6HisHEK293 cellsRP01766
Recombinant Rat IL-7 ProteinRat25647C-6HisHEK293 cellsRP01721
Recombinant Human IL-12/IL-12A&IL-12B ProteinHuman3592&3593C-His(IL12B)&C-Flag(IL12A)HEK293 cellsRP01232
Recombinant Mouse IL-12/IL-12A&IL-12B ProteinMouse16159&16160C-His(IL-12a)&No tag(IL-12b)HEK293 cellsRP01691
Recombinant Human IL-15 ProteinHuman3600C-HisE. coliRP01236
Recombinant Mouse IL-15 ProteinMouse16168C-6HisE. coliRP01676
Recombinant Human IL-18 ProteinHuman3606No tagE. coliRP00006
Recombinant Mouse IL-18 ProteinMouse16173C-HisHEK293 cellsRP02521
Recombinant Mouse IL-21 ProteinMouse60505C-HisCHO CellsRP02775S
Recombinant Rat IL-21 ProteinRat365769C-6HisHEK293 cellsRP01756

NK细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Human IL-2 stimulates cell proliferation of the CTLL2 cells.
Recombinant Mouse IL-2 stimulates cell proliferation of the CTLL2 cells
Recombinant Mouse IL-7 stimulates cell proliferation of the murine 2E8 cells
客户培养数据展示
南京中医药大学
Experiment Type
Cell-based assay
Sample
NK92
Product
Recombinant Human IL-2 Protein (RP01039)
Description
After NK92 cells were stimulated with 100 ng/mL IL-2 for 48 h, the number of clusters increased significantly and the activity was enhanced.

T细胞是适应性免疫能力的重要组成部分,可对病原体启动细胞介导的免疫应答,大致分为辅助性T细胞(CD4+T细胞)和细胞毒性T细胞(CD8+T细胞)。CD4+T细胞包含Th1、Th2、Th9、Th17、Th22、Treg和Tfh等不同的亚群,通过分泌细胞因子产生炎症或激活其他免疫细胞。CD8+T细胞按照分泌的细胞因子的不同分为Tc1,Tc2,Tc17, Tc9和Tc22等亚群,可直接杀死被病原体感染或癌变的细胞。每种T细胞亚群都由一组特定的细胞因子和转录因子活化,这些细胞因子在T细胞增殖、分化的培养过程中发挥了重要作用。

T细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Rat IL-1 alpha ProteinRat24493C-6HisHEK293 cellsRP01736
Recombinant Human IL-1 beta ProteinHuman3553C-HisE. coliRP00002
Recombinant Mouse IL-1 beta ProteinMouse16176C-HisE. coliRP01340
Recombinant Rat IL-1 beta ProteinRat24494C-HisHEK293 cellsRP01788
Recombinant Human IL-2 ProteinHuman3558C-HisHEK293 cellsRP01039
Recombinant Mouse IL-2 ProteinMouse16183C-HisHEK293 cellsRP01384
Recombinant Rat IL-2 ProteinRat116562C-HisHEK293 cellsRP01769
Recombinant Human IL-4 ProteinHuman3565C-HisHEK293 cellsRP00995
Recombinant Mouse IL-4 ProteinMouse16189C-HisHEK293 cellsRP01161
Recombinant Rat IL-4 ProteinRat287287C-HisHEK293 cellsRP01874
Recombinant Human IL-6 ProteinHuman3569C-HisHEK293 cellsRP00201
Recombinant Mouse IL-6 ProteinMouse16193C-HisHEK293 cellsRP01321
Recombinant Rat IL-6 ProteinRat24498C-HisHEK293 cellsRP01741
Recombinant Human IL-7 ProteinHuman3574C-HisHEK293 cellsRP01040
Recombinant Mouse IL-7 ProteinMouse16196C-6HisHEK293 cellsRP01766
Recombinant Rat IL-7 ProteinRat25647C-6HisHEK293 cellsRP01721
Recombinant Human IL-12/IL-12A&IL-12B ProteinHuman3592&3593C-His(IL12B)&C-Flag(IL12A)HEK293 cellsRP01232
Recombinant Mouse IL-12/IL-12A&IL-12B ProteinMouse16159&16160C-His(IL-12a)&No tag(IL-12b)HEK293 cellsRP01691
Recombinant Human IL-15 ProteinHuman3600C-HisE. coliRP01236
Recombinant Mouse IL-15 ProteinMouse16168C-6HisE. coliRP01676
Recombinant Mouse IL-21 ProteinMouse60505C-HisCHO CellsRP02775S
Recombinant Rat IL-21 ProteinRat365769C-6HisHEK293 cellsRP01756
Recombinant Human IL-23/IL-12B&IL-23A ProteinHuman3593&51561No tag(IL12B)&N-his(IL23A)HEK293 cellsRP01160
Recombinant Mouse IL-23/IL-12B&IL-23A ProteinMouse16160&83430N-His(IL-23a)&No tag(IL-12b)HEK293 cellsRP02928S
Recombinant Cynomolgus IL-23A&Mouse IL-12B ProteinCynomolgus&Mouse16160&102128555N-HisHEK293 cellsRP02725
Recombinant Human IL-33 ProteinHuman90865No tagE. coliRP00019
Recombinant Mouse IL-33 ProteinMouse77125No tagE. coliRP00618
Recombinant Human IFN-gamma ProteinHuman3458No tagE. coliRP01038
Recombinant Mouse IFN-gamma ProteinMouse15978C-hFc&HisHEK293 cellsRP01070
Recombinant Rat IFN-gamma ProteinRat25712C-hFc&HisHEK293 cellsRP01075
Recombinant Human TNF-alpha ProteinHuman7124C-HisHEK293 cellsRP00993
Recombinant Mouse TNF-alpha ProteinMouse21926C-HisHEK293 cellsRP01071
Recombinant Rat TNF-alpha ProteinRat24835C-HisHEK293 cellsRP01322
Recombinant Human Mature TGF-beta 1 ProteinHuman7040No tagHEK293 cellsRP01458
Recombinant Mouse/Rat Mature TGF-beta 1 ProteinMouse21803No tagHEK293 cellsRP00671
Recombinant Human CXCL13/BLC ProteinHuman10563No tagE. coliRP02839

T细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Human IL-2 stimulates cell proliferation of the CTLL2 cells.
Recombinant Mouse IL-2 stimulates cell proliferation of the CTLL2 cells
Recombinant Mouse IL-12 Protein induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells
客户培养数据展示
厦门大学
Experiment Type
Cell-based assay
Sample
T cell
Product
Recombinant Mouse IL-12/IL-12A&IL-12B Protein (RP01691)
Recombinant Human Mature TGF-beta 1 Protein (RP01458)
Recombinant Mouse IL-23/IL-12B&IL-23A Protein (RP02928S)(Upgraded product)
Description
A.Recombinant mouse IL-12 (Cat. RP01691) induced mouse Th1 differentiation. The expression of IFN-γ was detected by flow cytometry.
B.Recombinant mouse IL-23 (Cat. RP02928LQ) and human TGF-β (Cat. RP01458) induced mouse Th17 differentiation. The expression of IL-17α was detected by flow cytometry.
中山大学
Experiment Type
Cell-based assay
Sample
Memory T cell
Product
Recombinant Human IL-2 Protein (RP01039)
Recombinant Human IL-7 Protein (RP01040)
Recombinant Human IL-15 Protein (RP01236)
Description
Human Memory T cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), IL-7 (10 ng/mL, Cat. RP01040) and IL-15 (10 ng/mL, Cat. RP01236), and the cells were observed at 0h, 24h, 36h, 48h. T Cell growth was good and cell proliferation was obvious.

B细胞是一种能够对病原体启动抗体介导的免疫应答的适应性免疫细胞,可生成高亲和力抗体、形成免疫记忆、充当抗原呈递细胞,并分泌细胞因子。骨髓中的B细胞源自造血干细胞,由其分化为共同淋巴祖细胞,随之生成自然杀伤细胞、T淋巴细胞和B淋巴细胞。在B细胞的发育过程中,有多种细胞因子会影响其生长、存活和抗体类型转换,例如IFN-α和IFN-β主要参与B细胞的生成和选择,而IL-4和IL-6则影响B细胞优先分泌特定类别的抗体。

B细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human IL-2 ProteinHuman3558C-HisHEK293 cellsRP01039
Recombinant Mouse IL-2 ProteinMouse16183C-HisHEK293 cellsRP01384
Recombinant Rat IL-2 ProteinRat116562C-HisHEK293 cellsRP01769
Recombinant Human IL-4 ProteinHuman3565C-HisHEK293 cellsRP00995
Recombinant Mouse IL-4 ProteinMouse16189C-HisHEK293 cellsRP01161
Recombinant Rat IL-4 ProteinRat287287C-HisHEK293 cellsRP01874
Recombinant Human IL-6 ProteinHuman3569C-HisHEK293 cellsRP00201
Recombinant Mouse IL-6 ProteinMouse16193C-HisHEK293 cellsRP01321
Recombinant Rat IL-6 ProteinRat24498C-HisHEK293 cellsRP01741
Recombinant Human IL-7 ProteinHuman3574C-HisHEK293 cellsRP01040
Recombinant Mouse IL-7 ProteinMouse16196C-6HisHEK293 cellsRP01766
Recombinant Rat IL-7 ProteinRat25647C-6HisHEK293 cellsRP01721
Recombinant Human IL-10 ProteinHuman3586C-HisHEK293 cellsRP00093
Recombinant Mouse IL-10 ProteinMouse16153C-HisHEK293 cellsRP01465
Recombinant Rat IL-10 ProteinRat25325C-HisHEK293 cellsRP03252
Recombinant Mouse IL-21 ProteinMouse60505C-HisCHO CellsRP02775S
Recombinant Rat IL-21 ProteinRat365769C-6HisHEK293 cellsRP01756
Recombinant Human c-Kit ligand/KITLG/SCF ProteinHuman4254C-HisHEK293 cellsRP00124
Recombinant Mouse c-Kit ligand/KITLG/SCF ProteinMouse17311C-HisHEK293 cellsRP01055
Recombinant Rat c-Kit ligand/KITLG/SCF ProteinRat60427C-HisHEK293 cellsRP01794
Recombinant Human TNFSF11/RANKL/CD254 ProteinHuman8600N-HisHEK293 cellsRP00183
Recombinant Mouse TNFSF11/RANKL/CD254 ProteinMouse21943N-His&N-hFCHEK293 cellsRP02134
Recombinant Human TNFSF5/CD40 ligand/CD154 ProteinHuman959N-6HisHEK293 cellsRP01733
Recombinant Mouse TNFSF5/CD40 ligand/CD154 ProteinMouse21947N-6HisHEK293 cellsRP01734
Recombinant Human TNFSF13B/BAFF/CD257 ProteinHuman10673No tagE. coliRP00018

B细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Human IL-2 stimulates cell proliferation of the CTLL2 cells.
Recombinant Mouse IL-4 stimulates cell proliferation of the HT-2 cells
Recombinant Mouse IL-7 stimulates cell proliferation of the murine 2E8 cells
客户培养数据展示
复旦大学附属中山医院
Experiment Type
Cell-based assay
Sample
Primary B cell
Product
Recombinant Human IL-2 Protein (RP01039)
Recombinant Human TNFSF13B/BAFF/CD257 Protein (RP00018)
Description
Human primary B cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), BAFF (10 ng/mL, Cat. RP00018), IL-21 (20 ng/mL, Cat. RP02508), and the cells grew well and formed into clusters after activation.
厦门大学
Experiment Type
Cell-based assay
Sample
B cell
Product
Recombinant Mouse IL-4 Protein (RP01161)
Description
B cells were cultured using mouse IL-4 (1 ng/mL, Cat. RP01161) for 4 days. Obvious cluster-like growing cells could be observed, indicating that IL-4 provides the necessary signals for the proliferation and activation of B cells.