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Recombinant Mouse IFN-gamma Protein (RP01070)

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Recombinant Mouse IFN-gamma Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55 kDa.

Immobilized recombinant Mouse IFNGR1 at 0.2μg/mL (100μL/well) can bind recombinant Mouse IFNG,the EC50 of Mouse IFNG is 2.2ng/mL.

Stimulates mouse bone marrow-derived macrophages and peritoneal macrophages differentiation into M1 Macrophages with IFN-γ. The expression of the related biological markers TNF-a, Nos2, IL-1β, and IL-6 were detected to be significantly upregulated. (Customer Feedback Data)

Recombinant Mouse IFNG protein stimulates ZBP1 gene (ISGs) expression in mouse L-929 cells. 10 ng/mL of Mouse IFNG can effectively stimulate the expression of ZBP1 gene after 24h. (Customer Feedback Data)

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货号: RP01070
促销价：   ￥380
货    期：现货产品
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文献引用 (2)

折叠内容

Publishing research using RP01070? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse IFN-gamma Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Cys155) of mouse Interferon Gamma (Accession #NP_032363.1) fused with an Fc, 6×His tag at the C-terminus.
标签
C-hFc&His
纯度
> 92% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse IFNGR1 at 0.2 μg/mL (100 μL/well) can bind recombinant Mouse IFNG, the EC50 of Mouse IFNG is 2.2 ng/mL.
2.Recombinant Mouse IFN-gamma stimulates mouse bone marrow-derived macrophages and peritoneal macrophages differentiation into M1 Macrophages(classically activated macrophage). The expression of the related biological markers TNF-a, Nos2, IL-1β, and IL-6 was detected to be significantly upregulated.
3.Recombinant Mouse IFNG protein stimulates ZBP1 gene(ISGs) expression in mouse L-929 cells. 10 ng/mL of IFNG can effectively stimulate the expression of ZBP1 gene after 24h.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Interferon-gamma (IFN-gamma ) is a secreted protein which belongs to the type II interferon family. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. Interferon-gamma is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway.

基因ID
15978
Swiss Prot
P01580
别名
IFG;IFI;IFN gamma;Interferon Gamma;IFNG
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