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Recombinant Rat CSF-1/M-CSF Protein (RP01847)

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Recombinant Rat CSF-1/M-CSF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 40-60 kDa.

Recombinant Rat CSF-1/M-CSF stimulates cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 5.63-22.51 ng/mL, corresponding to a specific activity of 4.44×104~1.78×105 units/mg.

All(2)|
货号: RP01847
促销价：   ￥950
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详细信息

种属
Rat
表达宿主
HEK293 cells
描述
Recombinant Rat CSF-1/M-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ser255) of Rat CSF-1/M-CSF (Accession #NP_076471.3 ) fused with a His tag at the C-terminus.
标签
C-His
内毒素
<0.1EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Macrophage colony-stimulating factor (M-CSF), also known as colony-stimulating factor-1 (CSF-1), is a hemopoietic growth factor that regulates the survival, proliferation, differentiation and function of the cells of the mononuclear phagocyte lineage. M-CSF is produced by a variety of cell types and acts both locally and humorally in an autocrine and paracrine manner. Through differential mRNA splicing and posttranslational proteolytic processing and modification, M-CSF is expressed in three biologically-active isoforms: a secreted glycoprotein; a secreted proteoglycan; and a membrane spanning cell-surface glycoprotein.

基因ID
78965
Swiss Prot
Q8JZQ0
别名
Macrophage colony-stimulating factor 1; CSF-1; MCSF; Proteoglycan macrophage colony-stimulating factor; PG-M-CSF; Cleaved into: Processed macrophage colony-stimulating factor 1; Macrophage colony-stimulating factor 1 43 kDa subunit，Csf1; Csfm

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