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活性验证

Recombinant Mouse IL-13 Protein (RP01596)

文献引用 (2) 说明书 收藏

Recombinant Mouse IL-13 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Mouse IL-13 stimulates cell proliferation of the TF-1 Mouse erythroleukemic cells. The ED50 for this effect is 5.4-21.4 ng/mL, corresponding to a specific activity of 4.68×104~1.85×105 units/mg.

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货号: RP01596
促销价：   ￥950
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
12.52 kDa
Observed MW
15-35 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
<0.1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse IL-13 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser26-Phe131) of mouse IL-13 (Accession #NP_032381.1) fused with and a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell proliferation assay using TF-1 Mouse erythroleukemic cells.The ED50 for this effect is 5.4-21.4 ng/mL, corresponding to a specific activity of 4.68×104~1.85×105 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (2)

Publishing research using RP01596? Please let us know so that we can cite the reference in this datasheet.

背景信息

Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4R α , generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.

基因ID
16163
Swiss Prot
P20109
别名
Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13;IL13;Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13;IL13