Recombinant Human IL-18 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18 kDa.
Immobilized recombinant Human IL18 at 5μg/mL (100μL/well) can bind recombinant Human IL18BPa with a linear range of 0.156-1.372μg/mL.
Recombinant Human IL-18 induce IFN-gamma secretion by KG 1 human acute myelogenous leukemia cells in the presence of TNF-alpha(Catalog: RP00001). The ED50 for this effect is 1.44-5.76 ng/mL, corresponding to a specific activity of 1.7×105~6.9×105 units/mg.
Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils. In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells.IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst.In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis.
