Western blot analysis of lysates from PC14 cells using Phospho-c-Jun-S73 Rabbit pAb (AP0119). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-c-Jun-S73 Rabbit pAb (AP0119) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-c-Jun-S73 Rabbit pAb (AP0119) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-c-Jun-S73 Rabbit pAb (AP0119) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Western blot analysis of lysates from Rat lung, using IRAK4 Rabbit pAb (A6208) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using IRAK4 Rabbit pAb (A6208) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat heart using IRAK4 Rabbit pAb (A6208) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using IRAK4 Rabbit pAb (A6208) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human hepatobiliary duct using IRAK4 Rabbit pAb (A6208) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using IRF3 Rabbit pAb (A11118) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using IRF3 Rabbit pAb (A11118) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using IRF3 Rabbit pAb (A11118) at dilution of 1:100 (40x lens). Secondary antibody: ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS073) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg IRF3 antibody (A11118). Western blot was performed from the immunoprecipitate using IRF3 (A11118) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (A11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of various lysates using TBK1/NAK Rabbit mAb (A3458) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using TBK1/NAK Rabbit mAb (A3458) at a dilution of 1:750 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human testis tissue using TBK1/NAK Rabbit mAb (A3458) at a dilution of 1:750 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using TBK1/NAK Rabbit mAb (A3458) at a dilution of 1:750 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of MCF7 cells using TBK1/NAK Rabbit mAb (A3458,at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using TBK1/NAK Rabbit mAb (A3458,at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg TBK1/NAK Rabbit mAb (A3458). Western blot was performed from the immunoprecipitate using TBK1/NAK Rabbit mAb (A3458) at a dilution of 1:1000.
Western blot analysis of various lysates using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
Confocal imaging of HeLa cells using IKKβ Rabbit mAb (A19606,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Western blot analysis of lysates from HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:3000 dilution. HeLa cells were treated with Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Western blot analysis of various lysates using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells and C6 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631).NIH-3T3 cells were treated with Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.