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Recombinant Human EGF Protein (RP01030)

Recombinant Human EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Human EGF enhances EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.

Immobilized Human EGFR at 5 μg/mL (100 μL/well) can bind Human EGF with a linear range of 7-25ng/mL.

Recombinant Human EGF promotes the proliferation of BALB/c 3T3 mouse embryonic fibroblasts cells. The ED50 for this effect is typically 0.065-0.26 ng/mL, corresponding to a specific activity of 3.84×106-1.54×107units/mg.

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货号: RP01030
促销价:   ¥380
货    期:现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
33.01 kDa
Observed MW
40 kDa
标签
C-hFc&His
纯度
≥ 90 % as determined by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human EGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn 971-Arg 1023) of human EGF (Accession #NP_001954.2) fused with hFc, 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
1.Measured by its ability to stimulate EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.
2.Measured by its binding ability in a functional ELISA. Immobilized Human EGFR at 5 μg/mL (100 μL/well) can bind Human EGF with a linear range of 7-25 ng/mL. 3.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.065-0.26 ng/mL, corresponding to a specific activity of 3.84×106-1.54×107units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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客户数据及评论 (3)

折叠内容
厦门百脑慧医学研究有限公司Jul 282025
评分
Experiment Type
Cell-based Assay
Sample
prostate cancer cells
Description
The picture show culture of human prostate cancer cells using Wnt-3a (RP01618SLQ), Noggin (RP01237), FGF-10 (RP01140), bFGF (RP01042), RSPO1 (RP00071), and EGF (RP01030).
南京客户Jun 112025
评分
Experiment Type
Cell-based assay
Sample
tissue
Description
Animal tissues-derived intestinal stem cells were cultured in organoid culture medium containing factor combinations (WNT-3A, EGF, R-spondin-1, Noggin, and matrix gel), and then small intestinal organoids were formed.
中国中医科学院医学实验中心Oct 232024
评分
Experiment Type
Cell-based assay
Sample
tumor tissue
Description
Patient tissue-derived kidney tumor cells were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071 + 20 ng/mL FGF-10, Cat. RP01140 + 50 ng/mL EGF, Cat. RP01030), and then kidney tumor organoids were formed.

Publishing research using RP01030? Please let us know so that we can cite the reference in this datasheet.

背景信息

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin binding EGF like growth factor (HB_x005f

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