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VIM Knockdown 293T Cell Lysate, Heterozygous (RM01793)

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Western blot analysis of extracts from parental (Control) and VIM knockdown (KD) 293T cells, using VIM antibody (A11952) at 1:1000 dilution.

Genome sequence analysis of PCR products from parental (WT) and VIM knockdown (KD) 293T cells, using sanger sequencing.

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货号: RM01793
价 格：  4700.00
货 期： 现货产品
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详细信息

靶点
VIM
细胞系
293T
突变描述
VIM Knockdown 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:1bp insertion and 352bp deletion in exon1
Allele-2:368bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Store at -20℃
运输条件
4℃

背景信息

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

基因ID
7431
Swiss Prot
P08670
别名
CTRCT30; HEL113
研究领域