Confocal imaging of HT-1080 cells (treated with TNF-α) and HT-1080 cells (untreated) cells using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653, dilution 1:2100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.