种属
Human rhinovirus 14 (HRV-14)
纯度
≥ 90% as determined by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Supplied as 0.22 μm filtered solution in 25mM Tris,150mM NaCl,1mMDTT,0.5mM EDTA,50% glycerol,pH8.0
描述
Recombinant human rhinovirus type 14 3C/ HRV 3C protease is produced by E. coli expression system. The target protein is expressed with sequence (Gly1538-Gln1719) of human rhinovirus type 14 3C/ HRV 3C (Accession #NP_041009.1) fused with a His and GST tag at the N-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
（1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
Specific activity: Using Cleavage Control Fusion Protein as substrate, the amount of enzyme required to cleave 100 μg of substrate was defined as 1 active unit (U) in 1×HRV 3C Cleavage Buffer system at 4°C for 16 hours.
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
HRV 3C Protease encoded by human rhinovirus 14 is a highly purified recombinant cysteine protease with a His-tag. It recognizes the sequence Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP), cleaving between the Gln (Q) and Gly (G) residues. HRV 3C protease folds into two anti-parallel six-stranded β-barrels and the site cleft is located at the junction of the two β-barrels domains. The enzyme requires neither metal nor cofactors for activity. It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃, even retaining robust activity at 4℃. Thus, cleavage can be performed at low temperature to enhance the stability of the target protein. The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.
