纯度
≥ 95 % as determined by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
描述
Recombinant Serratia marcescens Nuclease Protein is produced by Pichia expression system. The target protein is expressed with sequence (Asp22-Asn266) of serratia marcescens Nuclease (Accession #WP_015377376.1) fused with no additional amino acid.
储存
Store at -70℃.This product is stable at ≤ -20℃ for up to 1 year from the date of
receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
（1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
1.One unit (U) is defined as the amount of enzyme required to change the absorption value of △A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.
2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37℃ for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.
