Recombinant Human ITK Kinase was resolved with SDS-PAGE under reducing (Lane 1) conditions.
The activity of ITK is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.
Tyrosine-protein kinase ITK/TSK also known as interleukin-2-inducible T-cell kinase or simply ITK, is a protein that in humans is encoded by the ITK gene. ITK is a member of the TEC family of kinases and is highly expressed in T cells. ITK contains the following domains, which are often found in intracellular kinases: N-terminus – PH (pleckstrin homology domain), TH – Tec family homology domain (including Bruton's tyrosine kinase Cys-rich motif and Proline rich region), SH3 – (Src homology 3), SH2 – (Src homology 2), C-terminus – tyrosine kinase, catalytic domain. ITK is thought to play a role in T-cell proliferation and differentiation, and is functionally important for the development and effector function of Th2 and Th17 cells. Mice lacking ITK were shown to not be susceptible to asthma.
