Recombinant Human CK1-delta/CSNK1D Kinase (RP03403LQ)

Recombinant Human CK1-delta/CSNK1D Kinase was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.

The activity of CSNK1D is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.

All(2)|

货号: RP03403LQ

详细信息

种属

Human

表达宿主

Baculovirus-Insect Cells

Calculated MW

73.8 kDa

Observed MW

60-80 kDa

标签

N-GST

纯度

≥ 85% as determined by SDS-PAGE;≥ 85% as determined by HPLC.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Supplied as a 0.22 μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, 5%glycerol, 5 mM DTT, 0.1 M Trehalose. (pH 7.5). Contact us for customized product form or formulation.

描述

Recombinant Human CK1-delta/CSNK1D Kinase is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Glu2-Arg415) of Human CSNK1D (Accession #P48730) fused with a N-GST tag.

储存

Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Aliquots below 10 μL are not advisable. Product must not be stored in diluted solutions. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

复溶

Please use running water to thaw it quickly.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

Casein kinase I isoform delta also known as CKI-delta or CK1δ is an enzyme that in humans is encoded by the gene CSNK1D. It is a member of the CK1 (formerly named casein kinase 1) family of serine/threonine specific eukaryotic protein kinases encompassing seven distinct isoforms (CK1α, γ1-3, δ, ε) as well as various post-transcriptionally processed splice variants (transcription variants, TVs) in mammalians. Like eukaryotic protein kinases (ePKs) the different isoforms of the CK1 family consist of a N-terminal and a C-terminal lobe (N- and C-lobe, respectively), which are connected via a hinge region. CK1δ is involved in the circadian rhythm, DNA damage and cellular stress, microtubule dynamics, cell cycle progression, genomic stability, mitosis and meiosis. CK1δ is also involved in the regulation of microtubule polymerization and stability of the spindle apparatus and centrosomes during mitosis by directly phosphorylating α-, β-, and γ-tubulin.

基因ID

1453

Swiss Prot

P48730

别名

CSNK1D; CK1δ; HCKID; CKI-delta; CKId; CK1d; Casein kinase I isoform delta

研究领域