纯度
≥ 90 % as determined by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
描述
Recombinant Human IL-24 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met 1-Leu 207) of Human IL-24 (Accession #NP_001172085.1) fused with His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
（1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL20 Rα and human IL20 Rβ. The EC50 for this effect is typically 0.05-0.25 ng/mL.
复溶
Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
Interleukin-24 (IL-24) also known as Melanoma differentiation-associatedgene 7 protein (MDA-7) is a member of the IL10 family of cytokines. IL24/MDA-7/IL24 can induce apoptosis selectively in various cancer cells.Overexpression of IL-24 leads to elevated expression of several GADDfamily genes, which correlates with the induction of apoptosis. Thephosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), andheat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced bythis gene in melanoma cells, but not in normal immortal melanocytes.Human IL-24/MDA-7/IL24 is secreted by activated peripheral bloodmononuclear cells and is the ligand for two heterodimeric receptors, IL22R1/IL-20R2 and IL-20R1/IL-20R2. Northern blot analysis revealed IL24/MDA-7/IL24 expression in human tissues associated with the immunesystem such as spleen, thymus, peripheral blood leukocytes and normalmelanocytes. IL-24/MDA-7/IL24 binding to either its endogenous receptorson human keratinocytes or to ectopically expressed receptors on babyhamster kidney cells leads to activation of the signal transducers andactivators of transcription.
