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活性验证

Recombinant Human EGF Protein (RP03287)

说明书 收藏

Recombinant Human EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Human EGF Protein is greater than 95% as determined by SEC-HPLC.

Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×106~2.50×107 units/mg.

Human stomach organoids organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Mouse large intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ)

Mouse small intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071).

All(6)|
货号: RP03287
促销价：   ￥380
货    期：现货产品
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详细信息

种属
Human
表达宿主
E. coli
Calculated MW
6.35 kDa
Observed MW
5-15 kDa
标签
No-Tag
纯度
≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by HPLC.
内毒素
< 0.01 EU/μg of the protein by LAL method
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Human EGF Protein is produced by E. coli Cells expression system. The target protein is expressed with sequence (Asn971-Arg1023) of Human EGF(Accession #NP_001954.2) fused with no tag .
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2ng/ml.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

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背景信息

As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor.

基因ID
1950
Swiss Prot
P01133
别名
EGF;Pro-epidermal growth factor; EGF; Cleaved into: Epidermal growth factor; Urogastrone
研究领域