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Recombinant Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein (RP03247)

Recombinant Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-135 kDa (ITGAX) and 85-95 kDa (ITGB2).

The purity of Human ITGAX&ITGB2 is greater than 95% as determined by SEC-HPLC.

Immobilized Human ITGAX&ITGB2, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 26.8 ng/mL determined by ELISA.

All(3)|
货号: RP03247
促销价:   ¥4000
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ((Phe20-Pro1107)(ITGAX)&(Gln23-Asn700)(ITGB2)) of human Integrin alpha X beta 2 (Accession #P20702-1&P05107-1) fused with His tag at the C-terminus.
标签
C-His
纯度
> 95% by Tris-Bis PAGE and HPLC.
内毒素
< 1.0 EU/μg of the protein by LAL method.
生物活性
Immobilized Human ITGAX&ITGB2, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 26.8 ng/mL determined by ELISA.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response.

基因ID
别名
αXβ2; CD11C; SLEB6; CD18; ITGAX&ITGB2; ITGA; ITGB2
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