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活性验证

Recombinant Rat Hepatocyte growth factor/HGF Protein (RP01776)

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Recombinant Rat HGF stimulates cell proliferation of the Mv.1.lu cells. The ED50 for this effect is 1.6-6.2 ng/mL, corresponding to a specific activity of 1.6×105~6.3×105 units/mg.

Recombinant Rat Hepatocyte growth factor/HGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP01776
价 格：  780.00
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详细信息

靶点
HGF
细胞系
80.14 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
80-100 kDa
产品组成
Gln 33-Leu728
物种
HEK293 cells
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

背景信息

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenicfactor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PANdomain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activatinga tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted bymesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability tostimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis,and tissue regeneration.

基因ID
24446
Swiss Prot
P17945
别名
HPTA；Hepatocyte growth factor；HGF

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