产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞系

活性验证

Recombinant Mouse EGF Protein (RP01684)

说明书 收藏 复制产品信息

Recombinant Mouse EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED50 for this effect is 0.16-0.63 ng/mL, corresponding to a specific activity of 1.59×106~6.25×106 units/mg.

Measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 20.3-81.2 pg/mL, corresponding to a specific activity of 1.23×107~4.93×107 units/mg.

All(3)|
货号: RP01684
价 格：  380.00
抗体定制
服务咨询 |
扫码下单
享受积分

详细信息

靶点
EGF
细胞系
6.45 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
11 kDa
产品组成
Asn977-Arg1029
物种
Pichia
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

背景信息

EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland.

基因ID
13645
Swiss Prot
P01132
别名
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone

最新浏览的产品