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Recombinant Human CD47 Protein (RP01306)

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Recombinant Human CD47 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-45 kDa.

Immobilized Human CD47 at 5 μg/mL (100 μL/well) can bind Human SIRPA with a linear range of 0.488-189.26 ng/mL.

Immobilized Human CD47 (Catalog: RP01306) at 2 μg/mL (100 μL/well) can bind Human SIRP-alpha/CD172a (Catalog: RP00171) with a linear range of 0.06-22.3 ng/mL.

Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(Catalog: RP00171) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (Catalog: RP01306) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).

All(4)|
货号: RP01306
促销价：   ￥620
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human CD47 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Pro139) of human CD47 (Accession #NP_001768.1) fused with an 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 5 μg/mL (100 μL/well) can bind Human SIRPA with a linear range of 0.488-189.26 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Human CD47 (Catalog: RP01306) at 2 μg/mL (100 μL/well) can bind Human SIRP-alpha/CD172a (Catalog: RP00171) with a linear range of 0.06-22.3 ng/mL.
3.Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(Catalog: RP00171) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (Catalog: RP01306) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

This protein is a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

基因ID
961
Swiss Prot
Q08722
别名
CD47; IAP; MER6; OA3; CD47 molecule;IAP;MER6;OA3
研究领域
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