Recombinant Human Noggin/NOG Protein (RP01237)

Human gastric cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), FGF10(Cat. RP01140), RSPO1(Cat. RP00071), Wnt-3a Surrogate(Cat. RP01618SLQ), A83-01(Cat. RPM0008), Y-27632 dihydrochloride(Cat.RPM0007), Gastrin Ⅰ(Cat. RPM0011), AB-27 Supplement(Cat. RPM0047LQ), N-2 Supplement(Cat. RPM0046LQ).

Human stomach organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Recombinant Human Noggin/NOG Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human colorectal cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237)， FGF10(Cat. RP01140), RSPO1(Cat. RP00071), HGF(Cat. RP01602), A83-01(Cat. RPM0008), SB202190(Cat. RPM0010), Y-27632 dihydrochloride(Cat. RPM0007), Acetylcysteine/NAC(Cat. RPM0017), Nicotinamide (Cat. RPM0016), Gastrin Ⅰ(Cat. RPM0011), AB-27 Supplement(Cat. RPM0047LQ).

Immobilized recombinant Human BMP2 at 1 μg/mL (100 μL/well) can bind Noggin with a linear range of 2-57 ng/mL.

Mouse lung organoids were cultured in Matrigel (Cat. RPM0003) with medium supplemented with EGF (Cat. RP03287), Noggin (Cat. RP01237), RSPO1 (Cat. RP00071), Wnt-3a (Cat. RP01618SLQ), and FGF-10 (Cat. RP01140). After 8 days of culture, the expression of lineage markers, including E-Cadherin (Cat. A20798), AQP5 (Cat. A23292), and CD49F (Cat. A26707), was assessed by ultra-plex immunofluorescence staining.

Mouse small intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071).

Immobilized recombinant Human BMP4 at 0.5 μg/mL (100 μL/well) can bind Noggin with a linear range of 4-47 ng/mL.

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 13.28-53.12 ng/mL in the presence of 50 ng/mL of recombinant human BMP-4, corresponding to a specific activity of 1.88×10⁴-7.53×10⁴ units/mg.

Mouse large intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ)

Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity.

Immobilized Human Noggin at 1 μg/mL (100 μL/well) can bind Noggin Rabbit pAb with a linear range of 1-3.5 ng/mL.

All(13)|

货号: RP01237

详细信息

靶点

Noggin/NOG

细胞系

23.89 kDa

突变描述

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

35-38 kDa

产品组成

Gln28-Cys232

物种

HEK293 cells

保存条件

Store at -20℃

运输条件

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

客户数据及评论 (5)

折叠内容

中国科学院动物研究所Nov 282025

评分
Experiment Type: Cell-based assay
Sample: hPSC
Description: The differentiation medium containing RSPO1 (500 ng/mL), Noggin (100 ng/mL), and EGF (100 ng/mL) was used to induce the differentiation of mid-hindgut cells into small intestinal organoids. After 7 days of induction, the expression of markers CDX2, MUC2, CgA, and E-Cad was assessed by immunofluorescence.
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厦门百脑慧医学研究有限公司Jul 282025

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Experiment Type: Cell-based Assay
Sample: prostate cancer cells
Description: The picture show culture of human prostate cancer cells using Wnt-3a (RP01618SLQ), Noggin (RP01237), FGF-10 (RP01140), bFGF (RP01042), RSPO1 (RP00071), and EGF (RP01030).
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南京农业大学Jun 112025

评分
Experiment Type: Cell-based assay
Sample: tissue
Description: Animal tissues-derived intestinal stem cells were cultured in organoid culture medium containing factor combinations (WNT-3A, Cat. RP01618+EGF, Cat. RP01030+R-spondin-1, Cat. RP01237+Noggin, Cat. RP00071+ matrix gel, Cat. RPM0003), and then small intestinal organoids were formed.
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复旦大学Nov 122024

评分
Experiment Type: Cell-based assay
Sample: Cell
Description: The picture shows the culture results of mESo (mouse esophageal organoids). R-Spondin1 (Cat. RP00071), Noggin (Cat. RP01237) and EGF (Cat. RP01502) were used during the organoids culture and the final concentration were 200，100，50 ng/mL respectively.
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中国中医科学院医学实验中心Oct 232024

评分
Experiment Type: Cell-based assay
Sample: tumor tissue
Description: Patient tissue-derived kidney tumor cells were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spondin-1, Cat. RP00071 + 20 ng/mL FGF-10, Cat. RP01140 + 50 ng/mL EGF, Cat. RP01030), and then kidney tumor organoids were formed.
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背景信息

By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.

基因ID

9241

Swiss Prot

Q13253

别名

NOG;SYM1;SYNS1;SYNS1A;noggin