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Recombinant Human Alpha-synuclein/SNCA (K23Q) Protein (RP00792)

货号: RP00792
促销价:   ¥5000
货    期:在线咨询货期
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详细信息

种属
Human
表达宿主
Calculated MW
Observed MW
标签
纯度
内毒素
制剂
描述
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. (1)未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周;
(2)干粉蛋白复溶浓度建议在0.1-0.5 mg/mL,不能低于0.1mg/mL,长期储存请添加保护剂(如0.1%BSA),并分装保存,避免反复冻融;
(3)液体蛋白的保存温度及效期请参考说明书,如对液体蛋白浓度有要求,请提前咨询销售或者技术支持。
生物活性

复溶
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich.