产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞系

活性验证

Recombinant Mouse Mature Beta-nerve growth factor/NGFB Protein (RP00667)

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Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.63-2.53 ng/mL, corresponding to a specific activity of 3.95×105~1.58×106 units/mg.

Recombinant Mouse Beta-nerve growth factor/NGFB Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP00667
价 格：  560.00
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详细信息

靶点
Beta-NGF
细胞系
12.4 kDa
突变描述
Lyophilized from a 0.2 μm filtered solution of 50mM NaAc,150mM NaCl,pH5.5.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
13 kDa
产品组成
Ser122-Gly241
物种
E. coli
保存条件
Store at -20℃
运输条件
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

背景信息

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophicfactor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knotfamily of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGFand 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection andrepair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is apotent neurotrophic factor that signals through its receptor β -NGFR, and plays a crucial role in thedevelopment and preservation of the sensory and sympathetic nervous systems.

基因ID
18049
Swiss Prot
P01139
别名
Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB