产品和服务 / 产品类型 / ELISA试剂盒 / ELISA 抗体对
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
No significant cross-reactivity or interference was observed.
Placed at 37°C for 7 days, the stability of the standard curve all conform to CV <10%.
* This package insert must be read in its entirety before using this product.
Mouse VEGFA Sandwich Immunoassay
| Recommended Concentration | Sample | ||
|---|---|---|---|
| ELISA | Capture | 1-4ug/mL | Rabbit anti-Mouse VEGFA mAb (Cat. No. RMK0564) |
| Detection | 0.02-0.08ug/mL | Rabbit anti-Mouse VEGFA mAb (Cat. No. RMK0565) | |
| Standard | 3.91-250pg/mL | Recombinant Mouse VEGFA Protein |
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
