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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
No significant cross-reactivity or interference was observed.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Human Monocyte Chemotactic Protein 1 Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.5-2ug/mL | Rabbit anti-Human Monocyte Chemotactic Protein 1 (CAP)(Cat. No.RM17902) |
| ELISA Detection | 0.0025-0.01ug/mL | Rabbit anti-Human Monocyte Chemotactic Protein 1 (DET)(Cat. No.RM17903) |
| Standard | 31.25-2000pg/mL | Recombinant Human Monocyte Chemotactic Protein 1 Protein |
Monocyte Chemotactic Protein-1 (MCP-1), also known as CCL2, MCAF and TDCF, is a heparin binding, 10-14 kDa member of the beta or CC family of chemokines. Currently, there are at least 26 human CC family members that generally range from 8-12 kDa in size. The majority of these chemokines are found on human chromosome 17 and all contain a typical three β-sheet/one α-helix structure. Human MCP-1 is synthesized as a 99 amino acid (aa) precursor that contains a 23 aa signal sequence coupled to a 76 aa mature region . The mature region contains a receptor binding and dimerization N-terminus plus a glycosaminoglycan (GAG)-binding C-terminus . MCP-1 undergoes O-linked but not N-linked glycosylation, and variability in the pattern of glycosylation accounts for variability in its MW . Heavily glycosylated forms of MCP-1 show increased half-life, while lightly glycosylated forms of MCP-1 show increased bioactivity . MCP-1 circulates as a monomer and is also suggested to form dimers and/or multimers . The monomer is considered the predominant form and demonstrates full chemotactic activity . Dimers and higher order forms are suggested to serve as a link between circulating cells and the surface of vascular endothelium. The mouse ortholog to human MCP-1 is termed JE, which contains a 49 aa extension at the C-terminus. Over their shared aa sequences, mature human MCP-1 and mouse JE share 57% aa sequence identity . Mature human and porcine MCP-1 aa sequences are 79% identical . Multiple isoforms of MCP-1 exist that are generated through proteolytic processing. Although MCP-1 is not subject to CD26/DPPIV N-terminal processing, it does undergo MMP processing by MMP-1, -2, -3, and -9 . Truncation at the N-terminus creates a 72 aa isoform (aa 28-99) that retains some bioactivity, a 71 aa isoform (aa 29-99) that shows no activity, and a C-terminally processed isoform (aa 24-92) that possesses full bioactivity . A wide variety of cells secrete MCP-1, including endothelial cells (EC), monocytes, fibroblasts, and vascular smooth muscle cells , mast cells , and astrocytes.
