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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Human CD274 Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 1-4ug/mL | Rabbit anti-Human CD274(CAP)(Cat. No.RM17855) |
| ELISA Detection | 0.05-0.2ug/mL | Rabbit anti-Human CD274(DET)(Cat. No.RM17856) |
| Standard | 25-1600pg/mL | Recombinant Human CD274 Protein |
CD274 ,also known as PD-L1 and B7-H1, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain. Within the ECD, human CD274 shares 96%, 73%, and 74% aa sequence identity with cynomolgus monkey, mouse, and rat CD274, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain. CD274 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells , keratinocytes , endothelial and intestinal epithelial cells , as well as a variety of carcinomas and melanoma.
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