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NDUFB6 Knockout HeLa Cell Lysate, Homozygous (RM02795)

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Genome sequence analysis of PCR products from parental (WT) and NDUFB6 knockout (KO) HeLa cells, using sanger sequencing.

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货号: RM02795
促销价：   ￥4700
货    期：现货产品
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详细信息

靶点
NDUFB6
细胞系
HeLa
突变描述
NDUFB6 Knockout cell line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:exon1 was deleted
Allele-2:exon1 was deleted

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.

基因ID
4712
Swiss Prot
O95139
别名
CI; B17
研究领域
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