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IGF1 Knockdown HeLa Cell Lysate, Heterozygous (RM02550)

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Genome sequence analysis of PCR products from parental (WT) and IGF1 Knockdown (KD) HeLa cells, using sanger sequencing.

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货号: RM02550
价 格：   ￥4700
货 期： 现货产品
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详细信息

靶点
IGF1
细胞系
HeLa
突变描述
IGF1 Knockdown HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:71bp deletion in exon2
Allele-2:WT

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

基因ID
3479
Swiss Prot
P05019
别名
IGF-I; IGFI; MGF
研究领域

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