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PROX1 Knockout 293T Cell Lysate, Homozygous (RM02498)

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Genome sequence analysis of PCR products from parental (WT) and PROX1 Knockout (KO) 293T cells, using sanger sequencing.

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货号: RM02498
价 格：  4700.00
货 期： 现货产品
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详细信息

靶点
PROX1
细胞系
293T
突变描述
PROX1 Knockout 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:190bp deletion in exon1
Allele-2:191bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Store at -20℃
运输条件
4℃

背景信息

The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

基因ID
5629
Swiss Prot
Q92786
研究领域

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