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SETD2 Knockdown 293T Cell Lysate, Heterozygous (RM02311)

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Genome sequence analysis of PCR products from parental (WT) and SETD2 Knockdown (KD) 293T cells, using sanger sequencing.

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货号: RM02311
促销价：   ￥4700
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详细信息

靶点
SETD2
细胞系
293T
突变描述
SETD2 Knockdown 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:203bp deletion in exon3
Allele-2:207bp deletion in exon3

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008]

基因ID
29072
Swiss Prot
Q9BYW2
别名
HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A; LLS; SET2; p231HBP
研究领域
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