Genome sequence analysis of PCR products from parental (WT) and MMP9 knockout (KO) 293T cells, using sanger sequencing.
Western blot analysis of extracts from parental (Control) and MMP9 knockout (KO) 293T cells, using MMP9 antibody at 1:1000 dilution.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
