产品中心 / 产品类型 / 细胞系&裂解液 / KO细胞裂解液

PARP1 Knockout HeLa Cell Lysate, Homozygous (RM01786)

说明书 联系我们 收藏

Genome sequence analysis of PCR products from parental (WT) and PARP1 knockout (KO) HeLa cells, using sanger sequencing.

All(1)|
货号: RM01786
促销价：   ￥4700
抗体定制服务咨询

文献引用 (0)

详细信息

细胞系
HeLa
突变描述
PARP1 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:exon2 was deleted
Allele-2:exon2 was deleted

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

基因ID
142
Swiss Prot
P09874
别名
ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1
研究领域
>