Note: Paraffin tissue sections, frozen tissue sections, cell slides (Frozen sections and cell slides need to be used with RM02984P antibody elution buffer dedicated for multiplex fluorescence staining).
The primary principle of this product is based on Tyramide Signal Amplification (TSA) technology. The TSA principle utilizes the peroxidase reaction of tyramide (i.e., HRP catalyzes H₂O₂ to activate fluorescently labeled tyramide salts, forming covalent binding sites), generating a substantial enzymatic reaction. The reaction product binds to surrounding protein residues (including tryptophan, histidine, and tyrosine residues), resulting in the deposition of a large amount of fluorophore at the antigen-antibody binding site to achieve signal amplification. Simultaneously, through antibody stripping, multiple fluorescence staining can be achieved using different fluorescent tyramides.
产品特点
1.High Sensitivity:Based on Tyramide Signal Amplification (TSA) technology, it offers ultra-high sensitivity and provides more accurate detection results.
2.Cost-Effective:Unlike traditional immunofluorescence, TSA allows the use of primary antibodies from the same or different species. Secondary antibodies are HRP-conjugated, and multiple dyes can be used for multiplex protein labeling.
3.High Stability:The fluorescence is resistant to quenching, ensuring extremely high product stability.
产品应用
Suitable for multiplex immunofluorescence experiments on paraffin sections,frozen sections,and cell slides.
