产品和服务 / 产品类型 / 生化试剂

TSA Fluorescence Double Staining Plus Kit(Rabbit secondary antibody){多重荧光免疫组化染色,双标三色,兔二抗} (RK50025P)

货号: RK50025P
价 格:  1200.00
抗体定制
服务咨询
|
扫码下单
享受积分

产品信息

产品简介
  • Note: Paraffin tissue sections, frozen tissue sections, cell slides (Frozen sections and cell slides need to be used with RM02984P antibody elution buffer dedicated for multiplex fluorescence staining).

  • The primary principle of this product is based on Tyramide Signal Amplification (TSA) technology. The TSA principle utilizes the peroxidase reaction of tyramide (i.e., HRP catalyzes H₂O₂ to activate fluorescently labeled tyramide salts, forming covalent binding sites), generating a substantial enzymatic reaction. The reaction product binds to surrounding protein residues (including tryptophan, histidine, and tyrosine residues), resulting in the deposition of a large amount of fluorophore at the antigen-antibody binding site to achieve signal amplification. Simultaneously, through antibody stripping, multiple fluorescence staining can be achieved using different fluorescent tyramides.
产品特点
  • 1.High Sensitivity:Based on Tyramide Signal Amplification (TSA) technology, it offers ultra-high sensitivity and provides more accurate detection results.
  • 2.Cost-Effective:Unlike traditional immunofluorescence, TSA allows the use of primary antibodies from the same or different species. Secondary antibodies are HRP-conjugated, and multiple dyes can be used for multiplex protein labeling.
  • 3.High Stability:The fluorescence is resistant to quenching, ensuring extremely high product stability.
产品应用
WB
产品组成
Product CodeProduct NameSize(10 T)Size(50 T)Storage Temperature
RK50025P-1 TYR-520 plus fluorochrome(200×) 5 μL(Concentrated type) 25 μL(Concentrated type) 2-8℃ , Protect from Light
RK50025P-2 TYR-570 plus fluorochrome(200×) 5 μL(Concentrated type) 25 μL(Concentrated type) 2-8℃, Protect from Light
RK50025P-3 TSA buffer 1.6 mL 8 mL 2-8℃
RK50025P-4 Polymer-HRP goat anti-rabbit secondary antibody(Ready-to-use) 1.6 mL 8 mL 2-8℃
RK50025P-5 DAPI staining solution(Ready-to-use) 1 mL 5 mL 2-8℃, Protect from Light
RK50025P-6 Antibody diluent 2 mL 10 mL 2-8℃
RK50025P-7 Peroxidase inhibitor solution 2 mL 10 mL 2-8℃, Protect from Light
RK50025P-8 Antifade mounting medium 1 mL 5 mL 2-8℃

注意事项

  • 1.Please read the instruction manual carefully before starting the experiment.
  • 2.This product is for Research Use Only. Not for use in diagnostic procedures or any other clinical applications.
  • 3.For your safety and health, please wear a lab coat and disposable gloves when handling this product.

保存条件和运输

Store at 2-8℃ for 12 months.

Publishing research using RK50025P? Please let us know so that we can cite the reference in this datasheet.

说明书

说明书下载