ABflo® 488 Annexin V/PI Apoptosis Detection Kit｛ABflo® 488 Annexin V/PI 细胞凋亡检测试剂盒｝ (RK05875)

This Kit is to detect the early stage apoptotic cell. Annexin V, a 35-36 kDa Ca2+ dependent phospholipid-binding protein has a high affinity for phospholipid phosphatidylserine (PS), and binds to cells with exposed PS. In apoptotic cells, the membrane PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing PS to the external cellular environment. Product name ABflo® 488 Annexin V/PI & Cat.No RK05875, can detect the exposed PS through flow cytometry or fluorescence microscope. Propidium Iodide (PI), ready-to-use nucleic acid dye, release red fluorescence light after DNA embedding. Although PI can not penetrate the entire membrane, it can penetrate necrotic cells or cells that have lost membrane integrity in the late stages of apoptosis. Therefore, use of both Annexin V and PI, cells that are considered viable are Annexin V negative and PI negative; cells that are in early apoptosis are Annexin V positive and PI negative; and cells that are in late apoptosis or already dead are are both ABflo® 488 Annexin V and PI positive.ABflo® 488 has excitation of the light in 491nm and emission of the light in 516nm. PI-DNA complex has maximum absorption in 535nm and maximum emission of the light in 615nm.

Apoptosis