ECL Basic Femto Kit (10fg), is a high-sensitivity chemiluminescent (ECL) HRP substrate that enables users to achieve femtogram-level protein detection (~1×10-13g) during Western blot analysis, with significant antibody conservation. This substrate delivers bright signals and femtogram-level detection sensitivity for Western blot experiments using horseradish peroxidase (HRP) conjugates. Compatible with a wide range of membranes, blocking buffers and antibody dilutions, it meets diverse Western blotting application requirements through outstanding performance, versatility and cost-effectiveness.
产品特点
1.High Sensitivity: Detects femtogram-level protein bands on nitrocellulose membranes or PVDF membranes.
2.Long Signal Duration: Under optimized conditions, blot bands incubated with the substrate can continuously emit detectable light signals for 6–8 hours.
3.Cost-Effective: Optimized formulation suitable for antibody detection at extremely low concentrations. Recommended dilutions:Primary antibody: 1:1,000–1:5,000 (starting from a 1 mg/mL stock solution) or 0.2–1 μg/mL;Secondary antibody: 1:20,000–1:100,000 (starting from a 1 mg/mL stock solution) or 10–50 ng/mL.
产品应用
Western Blot (the most core and widely used application),Immunoblotting and Hybridization Assays
产品组成
Composition
Size 1
Size 2
SolutionⅠ
50 mL
250 mL
SolutionⅡ
50 mL
250 mL
注意事项
1.Please read the instruction manual carefully before starting the experiment.
2.This product is for Research Use Only. Not for use in diagnostic procedures or any other clinical applications.
3.For your safety and health, please wear a lab coat and disposable gloves when handling this product.
