Flow cytometric analysis of mouse spleen tissue was performed using the following antibody panel: PE-conjugated rat anti-mouse Ly-6G (A27159), PE/Cyanine7-conjugated rabbit anti-mouse CD11b (A27339), APC/Cyanine7-conjugated rabbit anti-mouse CD45 (A26831), ABflo® 488-conjugated rat anti-mouse CD3 (A27161), ABflo® 647-conjugated rabbit anti-mouse CD8a (A24942), and ABflo® 500-conjugated rabbit anti-mouse CD4 (A27843). The analysis demonstrated clear separation between positive and negative populations within both T lymphocyte and neutrophil compartments.
Flow cytometric analysis of mouse heart tissue was performed using the following antibody panel: PE-conjugated rat anti-mouse Ly-6G (A27159), PE/Cyanine7-conjugated rabbit anti-mouse CD11b (A27339), APC/Cyanine7-conjugated rabbit anti-mouse CD45 (A26831), ABflo® 488-conjugated rat anti-mouse CD3 (A27161), ABflo® 647-conjugated rabbit anti-mouse CD8a (A24942), and ABflo® 500-conjugated rabbit anti-mouse CD4 (A27843). The analysis demonstrated clear separation between positive and negative populations within both T lymphocyte and neutrophil compartments.
Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M).
