Flow cytometry analysis of human PBMCs using ABflo® 594 Rabbit anti-Human CD62L/L-Selectin mAb (A24632), PE Mouse anti-Human CD3 mAb (A22795), ABflo® 488 Rabbit anti-Human/Monkey CD4 mAb (A22773), APC Rabbit anti-Human/Monkey CD8a mAb (A24799) and ABflo® 594 Rabbit anti-Human CD38 mAb (A24366) clearly classified positive and negative cells.
The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants.
