The figure shows the gating strategy for a blank tube (top) and an ABclonal antibody ABflo®647 Rabbit anti-Mouse CD95 mAb single-staining tube (bottom). The single-staining tube shows a clear separation of CD95+T cells from the negative cell population, with distinct boundaries that allow for a clear delineation of the CD95+T cell population for subsequent analysis.
CD95, also known as Fas, is a approximately 45 kD type I transmembrane protein in the TNFR superfamily (TNFRSF6). CD95 was expressed in thymus, spleen, liver, heart, lung, ovary and other organs. CD95 binds ligand (FasL) to form a death-inducing signaling complex (DISC) in cells to induce apoptosis. Cd95-induced apoptosis plays an important role in development and in maintaining peripheral tolerance of the immune system.
