Recombinant protein (or fragment).This information is considered to be commercially sensitive.
克隆号
ARC56633
产品形式
Liquid
偶联物
ABflo® 647. Ex:648nm. Em:664nm.
克隆号
ARC56633
产品形式
Liquid
偶联物
ABflo® 647. Ex:648nm. Em:664nm.
细胞定位
Lysosome, Plasma membrane, Endosome membrane.
存储缓冲液
Store at 2-8℃. Avoid freeze. Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
After adding PMA to stimulate THP1 cells and induce their differentiation into mature macrophages, they attached to the plate after 24 hours. Then, LPS was added to induce their polarization towards M1 macrophages after 6 hours, and the cells were cultured in 1640 medium with 10% serum for 48 hours. Using flow cytometry, the expression of M1 macrophage markers CD68 and CD86 was detected after polarization, and the experimental results were consistent with expectations.
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.
