
Western blot analysis of various lysates using DiMethyl-Histone H3-K79 Rabbit mAb (A22086) at 1:65000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): H3 proteinExposure time: 90s.

Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H3-K79 antibody (A22086) at 1:20000 dilution.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Confocal imaging of NIH/3T3 cells using DiMethyl-Histone H3-K79 Rabbit mAb (A22086, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of C6 cells using DiMethyl-Histone H3-K79 Rabbit mAb (A22086, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of paraffin-embedded Human spleen tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Confocal imaging of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K79 Rabbit mAb (A22086, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Immunoprecipitation analysis of 300 μg extracts of 293F cells using 3 μg DiMethyl-Histone H3-K79 antibody (A22086). Western blot was performed from the immunoprecipitate using DiMethyl-Histone H3-K79 antibody (A22086) at a dilution of 1:20000.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 Rabbit mAb antibody (A22086) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells and DiMethyl-Histone H3-K79 Rabbit mAb (A22086). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci (GAPDH), as shown in figure.