
Western blot analysis of various lysates using TROP-2 Rabbit mAb (A20824) at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophageal tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Confocal imaging of paraffin-embedded Human urothelial cancer tissue using TROP-2 Rabbit mAb (A20824, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

The multiplex IHC analysis on paraffin-embedded Human cervical cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : TROP-2 Rabbit mAb (A20824, 1:8000) with TSA-TYR-520 (Green), CD34 Rabbit mAb (A19015, 1:100) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

The multiplex IHC analysis on paraffin-embedded Human breast cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : TROP-2 Rabbit mAb (A20824, 1:8000) with TSA-TYR-520 (Green), CD34 Rabbit mAb (A19015, 1:100) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.