Observed MW
70-90 kDa due to glycosylation.
纯度
> 95% as determined by HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
描述
Recombinant Rabbit Coagulation factor XI Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu19-Pro624 ) of Rabbit Coagulation factor XI (Accession #G1SSQ6) fused with His tag at the C-Terminus. The protein needs to be activated by Thermolysin for an activated form.
储存
Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
（1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
Immobilized Rabbit Coagulation factor XI, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA (QC Test).
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis.
