纯度
> 95% as determined by Bis-Tris PAGE,
> 95% as determined by HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Supplied as 0.22μm filtered solution in 50mM Tris, 300mM NaCl, 1mM TCEP (pH 7.5).
描述
Recombinant Human AGR-2 Protein is produced by E. coli Cells system. The target protein is expressed with sequence (Arg21-Leu175) of Human AGR-2 (Accession # NP_006399.1) fused with His tag at the C-Terminus.
储存
Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
(1)未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周;
(2)干粉蛋白复溶浓度建议在0.1-0.5 mg/mL,不能低于0.1mg/mL,长期储存请添加保护剂(如0.1%BSA),并分装保存,避免反复冻融;
(3)液体蛋白的保存温度及效期请参考说明书,如对液体蛋白浓度有要求,请提前咨询销售或者技术支持。
生物活性
Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 μM as determined in SPR assay.
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells .
Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion.