纯度
> 95% as determined by Bis-Tris PAGE，
> 95% as determined by HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
描述
Recombinant Mouse CD5L Protein is produced by HEK293 Cells system. The target protein is expressed with sequence (Glu22-Val352) of Mouse CD5L (Accession #NP_033820.2) fused with His tag at the C-Terminus..
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Dissolve the lyophilized protein in distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine rich) group B family of proteins. CD5L is up-regulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. In the thymic cortex, CD5L protects cortical CD4+CD8+thymocytes from apoptosis. CD5L circulates in the serum in complex with IgM.
