Recombinant Human ZAP70 Kinase (RP03400LQ)

Recombinant Human ZAP70 Kinase was resolved with SDS-PAGE under reducing (Lane 1) conditions.

The activity of ZAP70 is based on the ADP-GLO kinase activity assay quantifies kinase activity by measuring the conversion of ATP to ADP catalyzed by the kinase. Specific reagents are used to convert the ADP in the reaction back to ATP, resulting in the production of a luminescent signal.

All(2)|

货号: RP03400LQ

详细信息

种属

Human

表达宿主

Baculovirus-Insect Cells

Calculated MW

97.5 kDa

Observed MW

85-100 kDa

标签

N-His-GST

纯度

≥ 90 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Supplied as a 0.22 μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 1 mM DTT. (pH 7.5). Contact us for customized product form or formulation.

描述

Recombinant Human ZAP70 Kinase is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Pro2-Ala619) of Human ZAP70 (Accession #P43403) fused with a N-His-GST tag.

储存

Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Aliquots below 10 μL are not advisable. Product must not be stored in diluted solutions. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

复溶

Please use running water to thaw it quickly.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

ZAP-70 (Zeta-chain-associated protein kinase 70) is a protein normally expressed near the surface membrane of lymphocytes (T cells, natural killer cells, and a subset of B cells). It is most prominently known to be recruited upon antigen binding to the T cell receptor (TCR), and it plays a critical role in T cell signaling. The T cell receptor has no innate enzymatic activity. Due to this, T cell receptors rely on signaling molecules to transduce a signal from the cell membrane. ZAP-70 is a critical cytoplasmic tyrosine kinase that initiates a signal pathway downstream of an activated T cell receptor. Due to its role in lymphocyte signaling, ZAP-70 has been associated with several diseases affecting lymphocytes. ZAP-70 expression is a significant indicator of the survival of lymphocytes and has been notably associated with chronic lymphocytic leukemia (CLL). CLL is a cancer that develops from overproduction of B cells in the bone marrow.

基因ID

7535

Swiss Prot

P43403

别名

ZAP70; SRK; Syk-related tyrosine kinase; 70 kDa zeta-chain associated protein; Tyrosine-protein kinase ZAP-70; ADMIO2; IMD48; STCD

研究领域