Recombinant Human PI3K/p110 delta&p85 alpha Kinase was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.
The activity of PK3CD/p85 is based on the ADP-GLO kinase activity assay quantifies kinase activity by measuring the conversion of ATP to ADP catalyzed by the kinase. Specific reagents are used to convert the ADP in the reaction back to ATP, resulting in the production of a luminescent signal.
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Phosphoinositide 3-kinases (PI3Ks), also called phosphatidylinositol 3-kinases, are a family of enzymes involved in cellular functions such as cell growth, proliferation, differentiation, motility, survival and intracellular trafficking, which in turn are involved in cancer. PI3K comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3CD also known as phosphoinositide 3-kinase (PI3K) delta isoform or p110δ. p110δ regulates immune function. In contrast to the other class IA PI3Ks p110α and p110β, p110δ is principally expressed in leukocytes. The Pik3r1 gene locus encodes the 85 kD regulatory subunit, as well as 55 and 50 kD regulatory subunits. It used to be thought that alternative splicing of this gene resulted in three transcript variants encoding different isoforms.
