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Active Recombinant Human PD-1/PDCD1/CD279 Protein (RP03166)

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Active Recombinant Human PD-1/PDCD1/CD279 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 64-68 kDa.

The purity of Active Recombinant Human PD-1/PDCD1/CD279 Protein is greater than 95% as determined by SEC-HPLC.

Immobilized Human PD-1, mFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA.

Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA.

All(5)|
货号: RP03166
促销价：   ￥150000
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human PD1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Leu25-Gln167) of Human PD1 fused with mFc at the C-terminal.
标签
C-mFc
纯度
> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
生物活性
1.Immobilized Human PD1 at 0.5 μg/mL(100 μL/Well). Dose response curve for Biotinylated Anti-PD1 Ab. with the EC50 of 68 ng/mL determined by ELISA.
2.Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.

基因ID
5133
Swiss Prot
Q8IX89 (Q15116)
别名
PDCD1; CD279; PD-1; PD1; SLEB2; hPD-1; hPD-l; hSLE1; programmed cell death 1;PD-1;CD279;PD1;SLEB2;hPD-1;hPD-l;hSLE1;PD-1/CD279
研究领域
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