纯度
≥ 95% as determined by Bis-Tris PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol pH 7.4.
描述
Recombinant Human TGM2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Ala687) of human TGM2 (Accession #) fused with a His tag at the N-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Immobilized Human TGM2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TGM2 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
Transglutaminase 2 (TGM2) is a member of the transglutaminase family, and it is reported to be associated with chemoresistance in various types of cancer. TGM2 was demonstrated to affect the chemosensitivity of osteosarcoma cells via regulation of the activation of mitogen‑activated protein kinase and AKT serine/threonine kinase pathways.