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Recombinant Cynomolgus IL-3RA/CD123 Protein (RP02637)

说明书 收藏

Recombinant Cynomolgus IL-3RA/CD123 Protein was determined by Tris-Bis PAGE under reducing conditions.

Cynomolgus IL-3 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-3, hFc Tag with an affinity constant of 48.13 nM as determined in SPR assay (Biacore T200).

Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).

All(3)|
货号: RP02637
促销价：   ￥4800
货    期：在线咨询货期
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详细信息

种属
Cynomolgus
表达宿主
HEK293 cells
Calculated MW
33.9 kDa
Observed MW
50-70 kDa
标签
C-His
纯度
≥ 95 % as determined by Tris-Bis PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
描述
Recombinant Cynomolgus IL-3RA/CD123 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg18-Arg302) of cynomolgus IL-3RA/CD123 (Accession #G8F3K3-1) fused with His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.

基因ID
102138639
Swiss Prot
G8F3K3-1
别名
CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha