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Recombinant SARS-CoV-2 Spike RBD(B.1.640.2/IHU) Protein (RP02104)

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SARS-Cov-2 Spike RBD (B.1.640.2/IHU) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-Cov-2 Spike RBD (B.1.640.2/IHU) is greater than 95% as determined by SEC-HPLC.

Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA.

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货号: RP02104
促销价：   ￥3000
货    期：在线咨询货期
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折叠内容

Publishing research using RP02104? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
SARS-CoV-2
表达宿主
HEK293 cells
描述
Recombinant SARS-CoV-2(2019-nCoV) Spike RBD(B.1.640.2/IHU)-His Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y)) of SARS-COV-2(2019-nCoV) Spike RBD(B.1.640.2/IHU)-His (Accession #QHD43416.1) fused with His tag at the C-terminus.
标签
C-His
纯度
>95% by SDS-PAGE;> 95% by HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
生物活性
Immobilized SARS-Cov-2 Spike RBD(B.1.640.2/IHU), His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3 ng/mL determined by ELISA.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

基因ID
43740568
Swiss Prot
QHD43416.1
别名
Envelope;SARS-CoV-2 Spike RBD (N501Y);Spike;Spike ECD;Spike RBD;Spike S1;Spike S2;Spike S2 ECD;S1-RBD protein;NCP-CoV RBD Protein;novel coronavirus RBD Protein;2019-nCoV RBD Protein;S glycoprotein Subunit1 RBD Protein
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