产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞系

活性验证

Recombinant Mouse FGF-10 Protein (RP01848)

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Recombinant Mouse FGF-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED50 for this effect is 3.66-14.64 ng/mL, corresponding to a specific activity of 56.83×104~2.73×105 units/mg.

All(2)|
货号: RP01848
价 格：  950.00
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详细信息

靶点
FGF-10
细胞系
19.54 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of 20mMPB,800mMNaCl，pH7.4

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
20-25 kDa
产品组成
Gln37-Thr209
物种
E. coli
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

背景信息

Fibroblast Growth Factor 10 (FGF 10) is an evolutionary conserved secreted growth factor mediating mostly mesenchymal to epithelial signaling. FGF 10 belongs to the FGF 7 subfamily and shares similar biochemical and amino acid sequences with its constituent members (FGF3, FGF 7 and FGF 22). As a paracrine FGF, FGF 10 elicits its biological responses by activating the fibroblast growth factor receptor 2b (FGF R 2b), is crucial for governing proximal distal outgrowth as well as patterning and acts upstream of the known apical ectodermal ridge (AER) marker FGF 8. FGF10 is also implicated in pancreatic cancer, and that overexpression of FGFR2b is associated with metastatic invasion.

基因ID
14165
Swiss Prot
O35565
别名
Fibroblast growth factor 10; FGF-10; Keratinocyte growth factor 2;Fgf10